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. 2020 Sep 14;28(2):715–729. doi: 10.1038/s41418-020-00618-6

Fig. 5. ARID2 enhances the methylation of IFN-γ promoter by recruiting DNMT1.

Fig. 5

a Relative luciferase activity of IFN-γ promoter in HDLECs following ARID2 overexpression or knockdown. b ChIP assay for the enrichment of ARID2 in the promoter region of IFN-γ in HDLECs. c ChIP-Re-ChIP assay to determine ARID2-DNMT1 co-occupancy in the promoter region of IFN-γ. d ChIP assay for the enrichment of DNMT1 in the promoter region of IFN-γ in HDLECs following ARID2 overexpression or knockdown. e Schematic map of the CpG island region in the IFN-γ promoter. f MSP assay to detect the methylation level of the IFN-γ promoter in HDLECs following ARID2 overexpression or knockdown. Error bars represent the mean ± SD of three independent experiments. ***P < 0.001.