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. 2021 Feb 4;12:796. doi: 10.1038/s41467-021-21005-w

Fig. 4. Utilization of 2′dGTP during processive transcription by the WT and variant E. coli RNAPs.

Fig. 4

a TECs were assembled using the scaffold shown above the gel panels and chased with 50 µM ATP, CTP, UTP, and GTP or 2′dGTP for 2 min at 25 °C. The positions of GMPs in resolved stretches of the transcribed sequence are marked along the right edge of gel panels; 16-bit grayscale scans were normalized using max pixel counts within each gel panel and pseudo-colored using RGB palette. b Lane profiles of transcription in all-NTPs and 2′dGTP chases by the WT (cyan) and β′R425K (magenta) RNAPs quantified from gels in a. Traces were manually aligned along the X-axis and scaled along the Y-axis using several sequence positions as references. All experiments were repeated in triplicate with similar results. Source data are provided as a Source Data file.