Table 2.
Kinetic parameters for the 2′dGTP utilization by E. coli RNAPs (fit to equation 1).
| RNAP | k, s−1 | β (stretching parameter) | Km, µM | Median reaction time, s | Median reaction rate, s−1 |
|---|---|---|---|---|---|
| WT | 0.62 (0.58–0.65) | 0.83 (0.80–0.85) | 190 (170–220) | 1.04 (0.98–1.11) | 0.67 (0.62–0.71) |
| β′R425K | 9.7 (8.4–11.3) | 0.90 (0.84–0.96) | 470 (370–590) | 0.069 (0.059–0.079) | 10.1 (8.6–11.5) |
| β′M932A | 1.01 (0.94–1.09) | 0.85 (0.81–0.89) | 160 (140–190) | 0.64 (0.59–0.69) | 1.07 (0.99–1.15) |
| β′Q929M | 0.071 (0.066–0.076) | 0.76 (0.73–0.79) | 112 (93–133) | 8.7 (8.1–9.3) | 0.080 (0.074–0.085) |
| β′N458S | 0.42 (0.39–0.45) | 0.83 (0.80–0.87) | 260 (220–310) | 1.5 (1.4–1.7) | 0.45 (0.41–0.49) |
Upper and lower bounds were calculated at a 10% increase in Chi2 by Origin 2015 software.