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. 2021 Jan 22;11:603298. doi: 10.3389/fpls.2020.603298

FIGURE 2.

FIGURE 2

Subcellular localization of different domains of CgbHLH001. (A) Prediction of NLS sequences of CgbHLH001. (B) Schematic diagram of different deletions of CgbHLH001 domains and the construction of the plant expression vector for subcellular localization. The green, orange, and blue regions represent N terminal, basic helix–loop–helix, and C terminal domains, respectively. (C) BiFC assay testing the localization. CBL: calcineurin B-like protein, membrane marker; GFP: green fluorescence protein; bright field: visible light; overlap: merged bright field with CBL and GFP. bHLH-GFP: full length of CgbHLH001 fused to GFP; 1–146 bHLH: 146 aa of CgbHLH001 N terminus; 1–197 bHLH: 197 aa of N terminus + bHLH domain of CgbHLH001; 147–197 bHLH: 147–197 aa bHLH domain of CgbHLH001; 147–263 bHLH: 147–263 aa bHLH domain + C terminus of CgbHLH001; 198–263 bHLH: 198–263 aa C terminus of CgbHLH001. Bar = 20 μm.