Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 22;14:586108. doi: 10.3389/fnins.2020.586108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Contino, Suelves, Vrancx, Vadukul, Payen, Stanga, Bertrand and Kienlen-Campard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Evaluation of mitochondrial morphology and content in PS2KO neurons. (A) Mitochondria from wild-type non-infected (Ct) and PS2KO neurons at DIV11 were stained with an anti-TOM20 antibody (green) and mature neurons were identified with MAP2 (red). Confocal microscope images were acquired and TOM20 images were processed using the MiNA toolset to generate accurate mitochondrial skeletons for further analysis. Scale bar = 20 μm. (B) Quantification of individuals (counts) and networks (counts) are shown in the graphs. Results are presented as mean ± sem. Student's t-test (n = 55 from N = 3 independent experiments). (C) RT-qPCR analysis of mitochondrial DNA content (Cyt-b and Nd1 mitochondrial genes) in wild-type non-infected (Ct) and PS2KO neurons at DIV11. Results (mean ± sem) are expressed as percentage of the respective control (Ct) (min N = 4).