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. 2021 Feb 4;11:106. doi: 10.1038/s41398-021-01223-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Representative image of synaptoporin (green) and calbindin (red) expression in adult Cntn4^+/+ and Cntn4^-/- hippocampi. DAPI is in blue. The scale bars represent 250 µm. B Schematic representation of the adult mouse hippocampus. The rectangle indicates the area and location used for quantification of mossy fiber crossings in the SP of the CA3. Abbreviations: CA1 cornu ammonis, CA3a-c cornu ammonis 3a-c, DG dentate gyrus, SPB suprapyramidal bundle, IPB infrapyramidal bundle, SP stratum pyramidale, slm stratum lacunosum-moleculare. C Quantification of the length (left panel) and area size (right panel) of the IPB, SPB, and CA3 in Cntn4^+/+ and Cntn4^-/- mice showed no difference between genotypes. Analysis was performed on at least three sections per brain from Cntn4^+/+ and Cntn4^-/- mice (n = 6 mice per genotype) using unpaired Student’s t test. Data are presented as mean ± S.E.M. D Quantification of percentage of mossy fibers crossing the SP did not reveal a difference between Cntn4^+/+ and Cntn4^-/- mice. Analysis was performed on at least three sections per brain from Cntn4^+/+ and Cntn4^-/- mice (n = 6 mice per genotype) using unpaired Student’s t test. Data are presented as mean ± S.E.M. E Nissl-stained sections of Cntn4^+/+, Cntn4^+/-, and Cntn4^-/- mice demonstrated a significant difference in hippocampal surface areas between genotypes. F Tracing of hippocampal subsections revealed significant area differences across all regions. Analysis was performed on at least two sections per brain from Cntn4^+/+, Cntn4^+/-, and Cntn4^-/- mice (n = 4 mice per genotype) using unpaired Student’s t test and one-way ANOVA. Data are presented as mean ± S.E.M, p = 0.0004, 0.004, 0.04 (total hippocampus); p = 0.001, 0.003 (CA1); p = 0.01, 0.048 (CA3); p = 0.03, 0.004 (DG).