Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 4;11:106. doi: 10.1038/s41398-021-01223-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Neuron morphology analysis results for Cntn4^+/+, Cntn4^+/-, and Cntn4^-/- mouse hippocampus CA1. A Schematic representation of the hippocampus CA1 with labeled Bregma anterior-posterior. Adapted from Paxinos and Franklin, 2001. B Golgi staining in Cntn4^+/+ and Cntn4^-/- mouse hippocampus CA1 (left), exemplary tracings of pyramidal neurons (right). The scale bar represents 40 µm. The arrowheads show differences in basal neurite length. C, E Quantitative morphological results for the apical and basal dendrites respectively. p = 0.04, 0.03 (apical volume); p = 0.02, 0.007 (apical surface area); p = 0.04, 0.04 (basal total neurite length); p = 0.02 (basal dendritic complexity index); p = 0.02 (basal volume); p = 0.04, 0.04 (basal surface area). D, F Sholl plots indicate the distribution of respective apical and basal dendritic intersections and length at increasing distance from the center of the cell body. p = 0.004, 0.02 (apical Sholl intersection); p = 0.02, 0.05, 0.04 (basal Sholl intersection); p = 0.03 (basal Sholl lengths). Quantitative analysis was performed, in each area, on at least six slices in Cntn4^+/+, Cntn4^+/-, and Cntn4^-/- mice (n = 5 mice per genotype). Data are presented as mean ± S. E. M, n = 19 (Cntn4^+/+), n = 14 (Cntn4^+/-), n = 14 mice (Cntn4^-/-). G Schematic view of the five different spine morphology categories, A = thin; B = stubby; C = mushroom; D = abnormal (several types); E = double mushroom. Quantitative morphological data on the first 25 μm (50 μm to 75 μm) of Golgi-stained branches of the proximal part of the apical dendrite in pyramidal neurons of the CA1 hippocampus region in Cntn4^+/+, Cntn4^+/-, and Cntn4^-/- mice indicate a significant difference in H total number of spines (including all morphological categories), and I relative distribution of mushroom spines. Data are presented as mean ± S. E. M, n = 10 (Cntn4^+/+), n = 14 (Cntn4^+/-), and n = 8 mice (Cntn4^-/-), p = 0.04, 0.04 (total spine), p = 0.021, 0.029 (mushroom-type spines).