Table 1.

Six samples pooling tests results.

Sample name	Average Ct	∆Cta	Calculated starting templates quantity	Copies/µl
Pooled group # 2	26.08 ± 0.26	1.41	2459.16 ± 407.82	307.4 ± 50.98
CoV-2 positive #2	24.68 ± 0.06		5921.69 ± 219.45	740.21 ± 27.43
Pooled group # 3	26.08 ± 0.65	1.25	2562.31 ± 898.64	320.29 ± 112.33
CoV-2 positive #3	24.83 ± 0.09		5383.47 ± 295.71	672.93 ± 36.96
Pooled group #1	30.34 ± 0.42	1.57	169.82 ± 42.64	21.22 ± 5.33
CoV-2 positive #1	28.78 ± 0.06		445.73 ± 16.31	55.72 ± 2.04
Pooled group # 8	31.58 ± 0.45	1.86	78.09 ± 22.88	9.76 ± 2.86
CoV-2 positive #8	29.72 ± 0.02		246.25 ± 2.70	30.78 ± 0.34
Pooled group # 4	31.36 ± 0.14	0.50	87.74 ± 7.68	10.97 ± 0.96
CoV-2 positive #4	30.85 ± 0.14		120.44 ± 10.41	15.06 ± 1.30
Pooled group # 5	35.14 ± 0.31	1.38	8.14 ± 1.56	1.02 ± 0.20
CoV-2 positive #5	33.76 ± 0.32		19.47 ± 4.06	2.43 ± 0.51
Pooled group # 7	–	–	–	–
CoV-2 positive #7	36.06 ± 0.16		4.52 ± 0.47	0.57 ± 0.06
Pooled group # 6	–	–	–	–
CoV-2 positive #6	36.13 ± 0.30		4.37 ± 0.79	0.55 ± 0.10
Average ∆Ct		1.33 ± 0.46

^aThe difference between Ct values of the particular pooled group and corresponding positive sample.