Table 2.

Nine samples pooling test results.

Sample name	Average Ct	∆Cta	Calculated starting templates quantity	Copies/µl
Pooled group # 2	27.34 ± 0.24	2.62	1108.34 ± 164.61	138.54 ± 20.58
CoV-2 positive #2	24.72 ± 0.01		5749.46 ± 43.17	718.69 ± 5.40
Pooled group # 3	28.25 ± 0.37	2.59	630.24 ± 154.01	78.78 ± 19.25
CoV-2 positive #3	25.67 ± 0.05		3162.27 ± 92.19	395.28 ± 11.52
Pooled group #1	31.46 ± 0.31	1.80	83.05 ± 15.12	10.38 ± 1.89
CoV-2 positive #1	29.66 ± 0.16		256.22 ± 24.97	32.03 ± 3.12
Pooled group # 8	32.85 ± 0.24	2.85	34.31 ± 4.91	4.29 ± 0.61
CoV-2 positive #8	30.00 ± 0.14		205.49 ± 17.99	25.69 ± 2.25
Pooled group # 4	32.89 ± 0.14	2.59	33.36 ± 2.94	4.17 ± 0.37
CoV-2 positive #4	30.29 ± 0.18		171.58 ± 19.55	21.45 ± 2.44
Pooled group # 5	37.30 ± 1.72	3.01	2.69 ± 2.45	0.34 ± 0.31
CoV-2 positive #5	34.29 ± 0.37		13.99 ± 3.21	1.75 ± 0.40
Pooled group # 7	–	–	–	–
CoV-2 positive #7	35.71 ± 0.85		6.21 ± 3.60	0.78 ± 0.45
Pooled group # 6	–	–	–	–
CoV-2 positive #6	36.80 ± 0.06		2.83 ± 0.11	0.35 ± 0.01
	Average ∆Ct	2.58 ± 0.42

^aThe difference between Ct values of the particular pooled group and corresponding positive sample.