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. 2021 Feb 4;11:3003. doi: 10.1038/s41598-021-82084-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Methane emissions (g CH₄/d) of weaned calves were persistently reduced with supplementation of 3-NOP. Control calves are represented by the light blue dashed line with triangles. Calves supplemented with 3-NOP until 14 weeks of life are represented by the navy blue line with circles. The grey shaded area represents the post-weaning treatment period. Methane emissions were measured using the GreenFeed System (C-Lock, Inc.). Data are treatment means and bars represent SE (n = 8 in CONT; n = 10 in 3-NOP group up to week 23, n = 9 for the measurement at 46–49 weeks post-weaning (57–60 weeks of life) due to the removal of a 3-NOP heifer from the herd due to infertility as a result of being born a twin, i.e., a free-martin).