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. 2021 Feb 4;11:3003. doi: 10.1038/s41598-021-82084-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Co-occurrence of ruminal genera differed in CONT versus 3-NOP calves at different ages. Circles represent relationships (positive, blue circles; negative, red circles) between taxa abundance in CONT (A) and 3-NOP (B) calves, determined using a Spearman non-parametric rank correlation matrix. Larger circles represent stronger correlations. Anaerobic fungi are displayed in red, archaeal taxa in green, and bacteria in blue.