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. 2021 Feb 4;12(2):152. doi: 10.1038/s41419-021-03434-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The docking scores of the screened natural products. Barasertib was used as a positive control. Pink, Barasertib; red, the 23 nature products with a docking score less than −6. B MTS assay analysis of the cell viability of CAL27 cells with the 23 nature products treatment. C The chemical structure of Tanshinone IIA (Tan IIA). D The cartoon representation of the Tan IIA binding pocket in Aurora B. E Details of the binding mode of Tan IIA with Aurora B. The compound was shown as yellow sticks, while the proteins were depicted in cartoon representation with key residues labeled and demonstrated as gray sticks. Hydrogen bonds were shown as red dashed lines. F Tan IIA directly binds with Aurora B in CAL27 cells ex vivo. The ex vivo pull-down assay was performed as described in “Material and methods”, the beads were boiled and subjected to IB analysis. G The increase of ATP concentration inhibits the interaction between Tan IIA and Aurora B. H The in vitro kinase assay analyzes the effect of Tan IIA on Aurora B kinase activity. Barasertib was used as a positive control; n = 3 independent biological replications, one-way ANOVA; ***p < 0.001.