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. 2021 Feb 4;12(2):152. doi: 10.1038/s41419-021-03434-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A MTS assay analyzes the cell viability of hTERT-OME cells with Tan IIA treatment for various time points. B MTS assay analyzes the cell viability of CAL27 (left), SCC25 (middle), and SCC15 (right) cells with Tan IIA treatment for 24 h; n = 3 independent biological replications, one-way ANOVA. C Soft agar assay examines the colony formation of CAL27 (top), SCC25 (middle), and SCC15 (bottom) cells treated with Tan IIA; n = 3 independent biological replications, one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001. D–G Edu incorporation assay and quantitative analysis of the effect of Tan IIA on OSCC proliferation. (D) The representative image of Edu incorporation assay for CAL27 cells. (E–G) Quantitative analysis of Edu incorporated cells for CAL27 (E), SCC25 (F), and SCC15 (G); n = 3 independent biological replications, one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001.