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. 2021 Feb 4;12(2):152. doi: 10.1038/s41419-021-03434-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A IB analysis of the protein level of Aurora B and p-H3 in CAL27/CAL27-IR and SCC25/SCC25-IR cells. B The effect of irradiation (IR) on cell viability of CAL27/CAL27-IR cells. CAL27 and CAL27-IR cells were treated with 4 Gy IR, cell viability was examined 72 h later by MTS assay; ***p < 0.001. C The effect of IR on colony formation of CAL27/CAL27-IR cells. CAL27 and CAL27-IR cells were treated with 4 Gy IR, colony number was examined 2 weeks later; ***p < 0.001. D IB analysis of the protein level of Aurora B and p-H3 in CAL27-IR cells treated with Tan IIA (2 μM), IR (4 Gy), or a Tan IIA/IR combination. E and F The cell viability (E) and colony formation (F) of CAL27-IR cells treated with Tan IIA (2 μM), IR (4 Gy), or a Tan IIA/IR combination; ***p < 0.001. G and H Immunofluorescence (G) or IB (H) analysis of γH2AX in CAL27-IR cells treated with Tan IIA (2 μM), IR (4 Gy), or a Tan IIA/IR combination. Scale bar, 5 μm; ***p < 0.001. I IB analysis of apoptosis in CAL27-IR cells treated with Tan IIA (2 μM), IR (4 Gy), or a Tan IIA/IR combination. J In vivo tumorigenesis of CAL27 cells treated with vehicle control, Tan IIA, IR, or a Tan IIA/IR combination; ***p < 0.001. K In vivo tumorigenesis of CAL27-IR cells treated with vehicle control, Tan IIA, IR, or a Tan IIA/IR combination; ***p < 0.001; ns, not statistically significant.