Figure 7.

Clinical relevance of 4.1N loss-induced entosis, 14-3-3-dependent EMT and anoikis resistance in EOC. (A and B) 4.1N were associated with 14-3-3ζ, Bax and Snail molecules expression in 268 primary human EOC specimens. Scale bar, 100 μm. (C) Kaplan-Meier PFS curves of patients with EOC with low versus high expression of 14-3-3ζ, Bax and Snail; (P = 0.0002, P = 1.57 × 10⁻⁶ and P = 5.87 × 10⁻⁷, respectively); (D) Kaplan-Meier OS curves of patients with EOC with low versus high expression of 14-3-3ζ, Bax and Snail; (P = 0.001, P = 0.003 and P = 0.0001, respectively). (E) 4.1N and 14-3-3ζ expression at the protein level was detected by western blot analysis in nine EOC tissues. β-Actin was used as an endogenous control. (F) H&E stained sections of abdominal ascites containing examples of entosis structures, as indicated by arrows. Scale bars = 50 μm (left); Correlation between abdominal ascites slides entosis occurrence and corresponding tissues 4.1N IHC scores (n = 10, Spearman correlation; P = 0.023, r = −0.704) (right). (G) 4.1N mRNA expression was correlated positively with E-cadherin mRNA expression and negatively with 14-3-3ε, 14-3-3τ, Snail2, N-cadherin, MMP1, BCL2A1 and RhoA mRNA expression in published profiles of ESCC (n = 535, P < 0.05; TCGA database of ovarian carcinoma)