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. 2021 Jan 22;8:581545. doi: 10.3389/fcell.2020.581545

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Copyright © 2021 Zhang, Wang, Wang, Zhao, Han, Zhao and Qu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Low expression of miR-29b-3p in human bone marrow-derived mesenchymal stem cell (hBMSC)-derived extracellular vesicles (EVs) isolated from osteoporotic patients. (A) EVs were isolated from bone marrow samples of 35 osteoporotic patients (osteoporosis group) and 20 non-osteoporotic patients (normal group) during total hip arthroplasty (THA), and their morphology was observed by transmission electron microscopy (200 nm). (B) Western blot analyses of EV specific markers, CD63, TSG101, syntenin-1. (C) The expression of miR-29b-3p in hBMSC-derived EVs obtained from 35 osteoporotic patients (osteoporosis group) and 20 non-osteoporotic patients (normal group) was determined by real-time quantitative polymerase chain reaction (RT-qPCR). *p < 0.05 compared to normal.