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. 2021 Jan 22;8:581545. doi: 10.3389/fcell.2020.581545

FIGURE 5.

FIGURE 5

Extracellular vesicle (EV)-encapsulated miR-29b-3p potentiated osteogenic differentiation through blocking the suppressor of cytokine signaling 1 (SOCS1)/nuclear factor (NF)-κB pathway by inhibiting lysine demethylase 5A (KDM5A). (A) Mice femur image was acquired by micro-CT scanning. (B) Quantitative analysis of bone mineral density (BMD), trabecular bone volume/tissue volume (TBV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), and structural model index (SMI) of the mouse femur. (C) miR-29b-3p level in the EVs was determined by real-time quantitative polymerase chain reaction (RT-qPCR). (D) The expression of KDM5A, SOCS1, and NF-κB (p65, p-p65) in mouse bone tissues was detected by western blot analysis. (E) Osteogenic differentiation in mouse bone tissues was analyzed by alizarine red staining (200×). *p < 0.05 compared to the sham group, #p < 0.05 compared to the OVX + agomiR-NC group, &p < 0.05 compared to the OVX + agomiR-miR-29b-3p group.