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LPS activates pro-inflammatory mediators, NO. The release of NO in LPS-induced and LPS-preconditioned cells were measured using Griess assay. Bars represent mean data ± S.E.M from three independent experiments. Data were analyzed using one-way ANOVA, followed by a Bonferroni post-hoc test. Relative release of nitrite in LPS-induced cells were compared to the control. ****p < 0.0001 considered to be statistically significant.
