Figure 20.

Analysis of cytoplasmic and nuclear expression of NF-κB by western blot. The cytoplasmic (A). and nuclear (B). NF-κB in LPS-induced cells, LPS-pre-conditioned cells, and LPS-induced cells with caspase inhibitors from the cell lysates. The expressions were determined by western blot. Band densities were measured by densitometric analysis, and data for the expression of cytoplasmic and nuclear NF-κB was normalized to the control while the expression was expressed as fold change relative to beta-actin. Bars represent data from three independent experiments. NF-κB expression in LPS-induced cells, LPS-preconditioned cells, and LPS-induced cells with caspase inhibitors was compared to control. *p < 0.05 and **p < 0.01, as determined by one-way ANOVA with Bonferroni's correction.