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. 2021 Jan 22;9:631016. doi: 10.3389/fcell.2021.631016

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Copyright © 2021 Tang, Xu, Zhang, Shi, Zhang, Yang, Lin, Liu, Lin, Geng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of hypoxia on apoptosis of GCs cultured in vitro. GCs were cultured in vitro with and without hypoxia, and then apoptosis was examined. (A) Representative immunoblotting of caspase-3. (B) Densitometric quantification of cleaved caspase-3. (C) Representative images of flow cytometry using Annexin V-PI staining. (D) Percentages of GC apoptosis. Each value represents the mean ± SE. One-way analysis of variance (ANOVA) was used to analyze the data, followed by a Tukey’s multiple range test. N = 6. NS, no significance.