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. 2021 Jan 22;9:605875. doi: 10.3389/fcell.2021.605875

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Copyright © 2021 Huang, Jiao, Zhu, Ning, Ye, Li, Hu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characteristics of differentiation and muscle fiber types in C2C12 cells with Mdfi overexpression. (A) Representative images of differentiating wild-type and overexpressing Mdfi C2C12 cells on days 1, 3, 5, and 7 in white light or myosin immunofluorescent staining. Myosin: red, a molecular marker of myogenesis; DAPI: blue, cell nuclei; Merge: the C2C12 cells fused into primary myotubes are shown in the insets. (B) Quantitative diagram of the percentage of myosin-positive C2C12 cells. (C) MyHC I, MyHC IIa, and MyHC IIb immunofluorescence staining in WT and Mdfi-OE C2C12 cells, respectively. (D) Quantitative diagram of the percentage of MyHC I, MyHC IIa, and MyHC IIb-positive C2C12 cells. The results are presented as Mean ± SEM of three replicates for each group. Magnification 200×. The scale bar on the photomicrographs represents 25 μm.