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. 2021 Jan 22;9:605875. doi: 10.3389/fcell.2021.605875

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Copyright © 2021 Huang, Jiao, Zhu, Ning, Ye, Li, Hu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mdfi promotes the differentiation of C2C12 cells. (A) qPCR detection of myogenic marker genes on days 1, 3, 5, and 7 in both WT and Mdfi-OE C2C12 cells. (B) Western blot identified the protein of myogenic marker genes on days 1, 3, 5, and 7 after differentiation induction in both WT and Mdfi-OE C2C12 cells. (C) The relative protein levels were obtained through Western blot band gray scanning analysis. (D) Co-IP identified the interaction of Mdfi with Myod and Myog, respectively. *p < 0.05; **p < 0.01; ***p < 0.001. The results are presented as Mean ± SEM of three replicates for each group.