FIGURE 3.

Eriochrome cyanine (EC) staining of the rostral–caudal spread of the lesions shows that more severe lesions extend for considerable distances in the spinal cord and affect white matter areas. (A) Histological sections processed for EC are shown for the three groups at the lesion epicenter and at 0.2 and 0.4 mm rostral and caudal. The graphs in (B,C) show the area of spared white matter of the left side and quantification of the left to right ratio at all intervals. Rapa, the rapamycin group; Veh, the vehicle group; Sham, the sham group; L, left; R, right; Epi, epicenter; mm, millimeters. *P < 0.05 between the rapamycin-treated and vehicle-treated groups; ^†P < 0.05 between the rapamycin-treated and sham groups; ^‡P < 0.05 between the vehicle-treated and vehicle-treated groups.