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. 2021 Jan 22;13:574041. doi: 10.3389/fnmol.2020.574041

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Copyright © 2021 Liu, Li, Huang, Lin, Ji, Huang, Liu, Wu, Wu, Jiang, Ye and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rapamycin does not alter the neuronal damage after spinal cord injury (SCI). Immunostained sections of the injury epicenter of the spinal cord with NeuN + and SMI312 + are shown in (A). High-magnification images of the NeuN + cells on the ipsilateral and contralateral side are shown in (B). (C) The NeuN + cells were significantly decreased on the ipsilateral side at the injury epicenter in the rapamycin and vehicle groups. (D) Expression of NeuN on the contralateral side at the injury epicenter showed no significant differences among the three groups. WPI, weeks post-injury; Rapa, the rapamycin group; Veh, the vehicle group; Sham, the sham group. Scale bar = 200 μm (A) and 20 μm (B). ^∗∗∗P < 0.001, ^nsP > 0.05. Error bars are mean ± SEM.