FIGURE 1.

Operational principle of the platform, in vitro, and in vivo experiments. A, The isolation is carried out using a biocompatible parylene polymer membrane. B, Immunofluorescence staining for identification of CTC and WBC. C, In vitro test: for the U87 cell line, the capture efficiency was 86.0%, 87.0%, 86.5%, 86.0%, 91.3%, and 92.9%, respectively. For the U251 cell line, the capture efficiency was 80.0%, 77.0%, 81.5%, 78.6%, 84.3%, and 86.2%, respectively. Each test was repeated three times. D, In vivo test: a representative fluorescence in vivo imaging of a mouse xenograft and HE staining of its brain. E, Representative image of mouse derived STEAM+/DAPI+/CD14,16,45‐ CTC. Scale bar = 10 μm. F, Quantification of STEAM+ cells and GFP+ cells in blood samples from sham‐injected mice (n = 5) and xenografts (n = 5). Threshold: 2 STEAM+ CTC per mL PB