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. 2021 Feb 4;11:3126. doi: 10.1038/s41598-021-82356-4

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation of H7-HA specific nanobodies. (a) The number of clones screened and the number of H7 or H3/H7 specific Nbs identified are shown from either phage display or yeast display library selection (Fig S1). (b) Serological immune response in immunised alpaca to A/Anhui/01/2013 (H7N9). Samples were taken pre-immunisation and after each of three boosts. (c) Serological neutralising immune response in immunised alpacas using micro-neutralisation assays of A/Anhui/01/2013 (H7N9) NIBRG-268. A positive control sheep serum specific for the reverse genetics virus was used as a positive control.