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. 2021 Feb 4;11:3126. doi: 10.1038/s41598-021-82356-4

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© Crown 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HA structure showing nanobody epitope groups. Surface structure of hemagglutinin H7-HA [A(H7N9) A/Shanghai/02/2013, (PDB file 4LN6)] showing key residues for each epitope group using Pymol 2.3.1 software. The structure shows HA1 domain (light green), HA2 domain (grey), and key residues for each epitope group, Gp1a (red), Gp1b/1c/1d (pink), Gp2 (yellow), Gp3 (blue) and Gp4 (brown). HA2 helix is shown in yellow with Gp5 epitopes residues highlighted in brown on the helix and numbering for the HA stem starts at sequence ‘GLFG…’.Previously described antigenic sites A, C and D ⁴⁷ on group 2 HA are indicated with dotted lines.