Table 2.

Characterisation of nanobody binding affinity and head/stem specificity.

Nanobody	Affinity (KD)
	A/Brisbane/10/2007 (H3N2) HA02	A/Chick/Netherlands/2003 (H7N7) HA02	A/Shanghai/01/ 2013 (H7N9) HA02	A/HongKong/125/ 2017 (H7N9) HA11
NB7-01	-	< 0.1 nM	4.80 nM	0.83 nM
NB7-02	-	6.19 nM	17.3 nM	6.24 nM
NB7-03	-	24.80 nM	35.70 nM	27.45 nM
NB7-04	-	8.74 nM	26.9 nM	10.79 nM
NB7-05	-	140 nM	6.96 nM	> 1 µM
NB7-07	-	0.81 nM	4.31 nM	0.78 nM
NB7-08	-	1.26 nM	2.22 nM	15.48 nM
NB7-09	-	< 0.1 nM	0.38 nM	0.93 nM
NB7-10	-	24.0 nM	21.6 nM	46.24 nM
NB7-11	-	4.60 nM	58.8 nM	18.74 nM
NB7-12	-	1.05 nM	1.50 nM	3.72 nM
NB7-13	-	> 1 µm	–	> 1 µm
NB7-14	-	< 0.1 nM	2.36 nM	0.41 nM
NB7-15	-	0.42 nM	1.24 nM	0.77 nM
NB7-16	-	1.63 nM	9.98 nM	4.95 nM
NB7-17	-	1.43 nM	1.30 nM	2.40 nM
NB37X-01	#	-	#	-
NB37X-02	#	-	#	-
NB37X-03	#	-	#	-
NB37X-04	#	-	#	-
NB37X-05	#	-	#	-
NB37X-06	#	-	#	-

¹ HA1 is purified hemagglutinin head domain.

² HA0 is purified full length hemagglutinin.

^# binding seen but could not be fitted using 1:1 fitting model.

- no binding could be seen.