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. 2021 Feb 4;11:3199. doi: 10.1038/s41598-021-82905-x

Figure 6.

Figure 6

Expression levels of genes involved in the ESX-1 secretion system: espC, espD, esxB, and esxN. (a–d) THP-1 macrophages were infected with the M. tuberculosis reference strain H37Rv (H) or the MKR superspreader (M) for 4 h. The expression levels of various genes involved in the ESX-1 secretion system were quantified by qRT-PCR. 2-∆∆ct is used for normalisation and relative quantification. One-way ANOVA with Tukey's multiple comparison test was used to determine which pairs of expression levels were significantly different (*: p < 0.05; **: p < 0.01; and ***: p < 0.001).