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. 2021 Feb 4;11:3026. doi: 10.1038/s41598-021-81860-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the atrial/ventricular human iPSC Double Reporter Line. (A) Representative immunofluorescence images of day 30 cardiomyocytes from the double reporter system, MYL-2/tdTomato (red) and SLN/eCFP (cyan), immunostained for cTNT (green). (B) Representative calcium tracing and quantification of amplitude, time to peak, TD50, rise rate, decay rate, and beat rate of MYL-2/tdTomato (+) and SLN/eCFP(+) cells (day 30) from the double reporter system. (C) Representative action potential tracing of MYL-2/tdTomato (+) and SLN/eCFP(+) cells (day 30) from the double reporter system separately using voltage activated fluorescent dye and quantification of APD75, decay rate, and beat rate of MYL-2/tdTomato (+) and SLN/eCFP(+) cells (day 30) from the double reporter system.