Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 4;11:3021. doi: 10.1038/s41598-021-81920-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Passive Nucleo-cytoplasmic protein fluxes of proteins within the Nichoid artificial niche. (a) Representative sketch of FRAP experiment to study fluorescent protein translocation into the cell nucleus and a representative plot of the fluorescence intensity recovery curve. The software used to create the images are Microsoft PowerPoint 2018 (https://products.office.com/it-it/powerpoint) and OriginPro 2019 (https://www.originlab.com/origin). (b) Contour map of GFP concentration on xz-plane at different time points for cells grown into the Nichoid (from numerical simulation). The software used to create the images is Ansys 2019 R2 (www.ansys.com) (c) Comparison of recovery curves and mean fluxes at 22 s after photobleaching for cells grown in the Nichoid: numerical simulation (black dotted curve), FRAP experiments (red curve). (d) Contour map of GFP concentration on xz-plane at different time points for cell on Control cells (from numerical simulation). The software used to create the images is Ansys 2019 R2 (www.ansys.com) (e) Comparison of recovery curves and mean fluxes at 22 s after photobleaching for cells grown on a flat control substrate: numerical simulation (black dot), FRAP experiments (blue). The vertical axis of the graphs (d) and (e) shows the normalized fluorescence intensity for FRAP and normalized concentration for the numerical simulation.