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. 2021 Feb 4;11:3146. doi: 10.1038/s41598-021-82181-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro potato tuber sprouting assay results. Tuber buds of three different varieties (Lady Claire, Ditta and Agata) were treated with two different isolates of Flavobacterium sp. (AIT1165 and AIT1181). For evaluation, bud growth was assessed according to the first principal growth stages of the BBCH scale. Hereby, the first stage 00 is considered innate or enforced dormancy with no sprouting at all, followed by stages 01 and 02, which represent the beginning of sprouting when sprouts are visible with sizes up to < 1 mm and 2 mm, respectively. According to the BBCH scale, dormancy is broken at stage 03. When tuber buds treated with maleic hydrazine reached stage 01, the assay was ended, and the sum of all growth stages of each replicate was calculated. Afterwards, the average of two repetitions was visualized in a boxplot diagram. The boxplot diagram shows that treatments with both isolate cultures led to sprouting inhibition in comparison to the assay control. The assay control gibberellic acid represents an effective sprouting promoter, whereas maleic hydrazine was used as the negative control. Ten percent tryptic soy broth (used as the medium for culture of the isolates) and sterile water were used as neutral controls.