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. 2020 Aug 19;28(2):640–656. doi: 10.1038/s41418-020-00609-7

Fig. 6. RNF220 promotes the interaction between STAT1 and JAK1.

Fig. 6

a Immunoblot analysis of STAT1 that coimmunoprecipitated with FLAG-JAK1 in the presence and absence of RNF220 and K63-ubiquitin from lysates of HEK293T cells transfected with plasmids as indicated. (b) Immunoblot analysis of JAK1 that coimmunoprecipitated with WT STAT1 and STAT1 mutant (K110R) in the presence and absence of RNF220 from lysates of HEK293T cells transfected with plasmids as indicated. c, d Immunoblot analysis of JAK1 that coimmunoprecipitated with STAT1 in WT and Rnf220+/– BMDMs in response to A. baumannii infection (c) and IFN-β treatment (d) for indicated times. e Immunoblot analysis of STAT1 and RNF220 in Stat1–/– L929 cells transfected with WT and mutated STAT1 plasmids (K110R, Y701F/S727A), RNF220 plasmid and vector control by lentivirus as indicated. f Gene expression analysis of Cxcl9 and Cxcl10 in untreated and IFN-β treated cells in e. g Immunoblot analysis of phosphorylation of STAT1 in Stat1–/– L929 cells transfected with WT and mutated STAT1 plasmids (K110R, Y701F/S727A), RNF220 and vector control in e with IFN-β treatment. Data represent two independent experiments and are presented as mean ± SEM. **P < 0.01; ***P < 0.001; ****P < 0.0001.