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. 2021 Feb 4;11:3046. doi: 10.1038/s41598-021-82357-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Multilocus sequence type (MLST)-based minimal spanning tree of 1220 Streptococcus uberis isolated from bovine mastitis milk as per NCBI database¹⁰³ (as of 8 June 2020) to date. Coloured circles are Australian isolates from bovine origin (n = 240). The tree was calculated and generated using the goeBURST version 1.2.1 (http://www.phyloviz.net/goeburst/)¹¹³ full MST algorithm in Phyloviz version 2.0 (www.phyloviz.net)¹¹². Node sizes reflect the number of isolates with specific MLST profile. Numbers within the nodes indicate the ST. Node colours refer to types of Global Clonal Complexes (GCC): GCC Strain Type (ST) 86 (green; left side of the plot), GCC ST143 (orange, right-bottom side of the plot), GCC ST5 (black, right-top side of the plot), isolates from Queensland are in red colour and Victorian isolates are in blue. Dashed rectangle show the location of the seven isolates to their possible ST founder ST251 (asterisk).