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. 2021 Feb 1;12(2):139. doi: 10.1038/s41419-021-03431-2

Fig. 6. GSDME knockdown relieved cisplatin-induced cell injury and pyroptosis in human TECs.

Fig. 6

Human TECs (HK-2) were transfected with FL-GSDME siRNA or the negative control and subsequently treated with cisplatin (10 μg/mL) for 24 h. a Protein level of FL-GSDME in human TECs after transfection with FL-GSDME siRNA (n = 3). b Transcriptional levels of KIM-1 (n = 3). c Representative bright-field microscopic images of cells. Red arrowheads indicate large bubbles from the plasma membrane (scale bar = 50 μm). d Role of GSDME silencing on LDH release (n = 6). e PI staining of cells with GSDME knockdown (scale bar = 100 μm; n = 3). f Role of GSDME siRNA on cell viability (n = 6). g IL-1β and IL-6 release in cells (n = 3). h Effect of GSDME siRNA on p-p65 protein levels (n = 4). *P < 0.05, **P < 0.01, and ***P < 0.001.