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. 2021 Feb 1;12(2):139. doi: 10.1038/s41419-021-03431-2

Fig. 7. Caspase-3-specific inhibitor Z-DEVD-FMK relieved cisplatin-induced renal tubular cell injury and pyroptosis.

Fig. 7

Human TECs (HK-2) and mouse kidney tissues subjected to Z-DEVD-FMK were used to determine the role of caspase-3 in GSDME cleavage and AKI progression. a Protein levels of FL-GSDME, GSDME-N, caspase-3, and cleaved caspase-3 detected by immunoblotting (n = 3). b Effect of Z-DEVD-FMK on LDH release (n = 6). c Representative bright-field microscopic images (scale bar = 50 μm) of cells. Red arrowheads indicate large bubbles from the plasma membrane. d Serum BUN of AKI mice subjected to Z-DEVD-FMK (n = 8). e Serum Cr of AKI mice subjected to Z-DEVD-FMK (n = 8). f PAS staining (scale bar = 50 μm) and associated renal tubular injury score of mice administered with daily injection of Z-DEVD-FMK (n = 8). *P < 0.05, **P < 0.01, and ***P < 0.001.