Skip to main content
. 2021 Jan 22;10:585452. doi: 10.3389/fonc.2020.585452

Figure 1.

Figure 1

Selection of OXA-resistant hepatocellular carcinoma (HCC) cells and establishment of cell lines in which RBM8A was overexpressed or knocked down. (A) Real time (RT)-PCR and western blot analysis of RBM8A expression in HCC cell lines. Western blot results were quantitated. (B) Schematic representation of the protocol used to obtain OXA-resistant HCC cells from the parental cell line (PCL). During concentration-elevation and intermittent induction treatment with OXA, each dose was maintained for 15 days. OXA-resistant cell lines were obtained by the end of 6 months. (C) Representative phase contrast images of Bel7404 PCLs and drug-resistant cells (DR-HCC cells, left panels) or MHCC97H PCLs and DR-HCC cells (right panels). Magnification, 20×. Scale bar, 20 μm. (D) Knockdown (KD) and overexpression (OE) efficiency of RBM8A in PCLs and DR-HCC cells based on RT-PCR and western blot analysis, compared with the negative control (NC). Western blot data were quantitated (right panels). Data were expressed as mean ± SD of three independent experiments, or were representative of three independent observations.