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. 2021 Jan 22;10:585452. doi: 10.3389/fonc.2020.585452

Figure 3.

Figure 3

Modulation of RBM8A expression affects the migratory and invasive potential of parental cell lines (PCLs) and drug-resistant (DR)-hepatocellular carcinoma (HCC) cells, as well as the expression of proteins related to drug resistance. (A) Wound-healing assay. The scraped areas were photographed at 0, 24, and 72 h after scraping. Migration efficiency was quantitated at 24 and 72 h after scraping (right panel). Magnification, 10×. Scale bar, 200 μm. (B) Transwell assay. Representative examples of each experimental group are shown. Migration efficiency was quantitated at 24 and 72 h (right panel). Magnification, 40×. Scale bar, 50 μm. (C) Matrigel-Transwell assay. Representative photographs and quantitation were shown. Data were either representative of three similar observations, or were shown as the mean ± SD of three experiments. Magnification, 40×. Scale bar, 50 μm. (D) Western blot analysis of PCL-Bel7404-NC, PCL-Bel7404-RBM8A-KD, DR-Bel7404-NC and DR-Bel7404-RBM8A-KD. Cells were analyzed without OXA treatment (second row) or with OXA treatment (third row). Data were representative of three similar observations or were shown as the mean ± SD of three experiments. (E) Western blot analysis of PCL-MHCC97H-NC, PCL-MHCC97H-RBM8A-OE, DR-MHCC97H-NC and DR-MHCC97H- RBM8A-OE cells as in (D).