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. 2021 Jan 22;11:616922. doi: 10.3389/fmicb.2020.616922

TABLE 1.

List of 40 fungi with the descriptions of methylation data.

Division Subphylum Order Organism Method Stage ave. 5mC mCG mCHG mCHH Mappability (%) References
Basidomycota Agaricomycotina Agaricales Coprinopsis cinerea WGBS Mycelia 3.03% 7.8% 0.6% 0.6% 89 Zemach et al., 2010
Laccaria bicolor WGBS Mycelia 3.73% 10.1% 0.5% 0.6% 57 Zemach et al., 2010
Agaricus bisporus HPLC Mycelia; yeast-like cells 4.01% NA NA NA NA Binz et al., 1998
Schizophyllum commune MSRE Mycelia NA NA NA NA NA Specht et al., 1984; Buckner et al., 1988
Armillaria bulbosa HPLC Mycelia; yeast-like cells 4.34% NA NA NA NA Binz et al., 1998
Pleurotus eryngii Subsp. Tuoliensis MSRE-PCR Mycelia NA NA NA NA NA Hua et al., 2017
Pleurotus eryngii var. eryngii WGBS Mycelia NA 16.40% 1.30% 1.50% 88.6 Zhang et al., 2018
Pleurotus tuoliensis WGBS Mycelia NA 14.60% 1.40% 1.70% 86.7 Zhang et al., 2018
Pleurotus ostreatus WGBS Mycelia PC 15 (n) NA 2.76% 0.38% 0.45% 72 Borgognone et al., 2018
Mycelia PC 9 (n) NA 4.37% 0.62% 0.84% 59
Mycelia N001-HyB (n + n) NA 3.96% 0.51% 0.65% 61
Mycelia M_N001 (n + n) NA 6.48% 0.45% 0.59% 64
Primordia NA 6.69% 0.43% 0.54% 59
Mature fruitbodies NA 6.54% 0.40% 0.51% 63
Russulales Heterobasidion parviporum WGBS Conidiospores (SPORE) 3.93% 8.43% 2.40% 2.20% 87.02 Zeng et al., 2019
Mycelia (MYCEL) 3.53% 7.23% 2.47% 2.10% 88.36
Saprotrophic growth (SAP) 4.33% 8.03% 3.10% 3.00% 88.18
Necrotrophic growth (NECT) 4.57% 8.30% 3.27% 3.17% 88.65
Polyporales Postia placenta WGBS Mycelia 3.10% 4.9% 1.2% 1.0% 80 Zemach et al., 2010
Ganoderma sinense WGBS Mycelia 4.64% 10.2% 0.8% 1.0% 77 Zhu et al., 2015
Sporotrichum dimorphosporum RE-NNA Mycelia 0.20% NA NA NA NA Antequera et al., 1984
Ustilaginomycotina Ustilaginales Ustilago maydis HPLC Mycelia; yeast-like cells 2.26% NA NA NA NA Binz et al., 1998
Ustilago violaceae HPLC Mycelia; yeast-like cells 2.14% NA NA NA NA Binz et al., 1998
Ascomycota Pezizomycotina Diaporthales Cryphonectria parasitica WGBS Mycelia 3.90% 0.09% 4.30% 4.30% 71.9 So et al., 2018
Eurotiales Aspergillus flavus WGBS Mycelia NA NA NA NA Liu et al., 2012
Magnaporthales Magnaporthe oryzae WGBS Conidia 0.613% 0.6% 0.6% 0.6% 83 Jeon et al., 2015
Mycelia 0.554% 0.6% 0.5% 0.5% 82
Appressoria 0.553% 0.5% 0.5% 0.6% 79
Sordariales Neurospora crassa GC/MS Conidia 0.36% NA NA NA NA Russell et al., 1987
Conidial germlings 0.40% NA NA NA NA
Early exponential mycelia 0.24% NA NA NA NA
Mid-exponential mycelia 0.24% NA NA NA NA
Stationary-phase mycelia 0.40% NA NA NA NA
Neurospora crassa WGBS Vegetativea 2.19% 2.08% 2.05% 2.19% 80.05 Hosseini et al., 2020
Pre-sexualb 2.86% 2.67% 2.44% 2.98% 77.35
Neurospora sitophila WGBS Vegetative 1.77% 1.67% 1.83% 1.72% 80.5
Pre-sexual 1.79% 1.68% 1.85% 1.75% 82.1
Neurospora tetrasperma L1 WGBS Vegetative 1.28% 1.23% 1.59% 1.13% 82.325
Pre-sexual 1.41% 1.31% 1.67% 1.29% 80
Neurospora tetrasperma L10 WGBS Vegetative 1.29% 1.30% 1.61% 1.13% 84.025
Pre-sexual 1.40% 1.37% 1.66% 1.28% 86.125
Neurospora tetrasperma L6 WGBS Vegetative 1.21% 1.25% 1.59% 1.00% 77.05
Pre-sexual 1.24% 1.27% 1.63% 1.03% 76.775
Hypocreales Cordyceps militaris WGBS Mycelia 0.48% 0.47% 0.48% 0.49% NA Xin et al., 2019
Fusarium oxysporum RE/Southern blot Mycelia NA NA NA NA NA Kim, 1997
Metarhizium robertsii WGBS Conidia 9d 0.403% 0.4% 0.4% 0.4% 93 Li et al., 2017
Conidia 15d 0.394% 0.4% 0.4% 0.4% 93
Mycelia 0.392% 0.4% 0.4% 0.4% 91
Metarhizium anisopliae WGBS Mycelia 0.60% 0.40% 0.45% 1.85% 85.17 Sbaraini et al., 2019
Onygenales Uncinocarpus reesii WGBS Mycelia 0.68% 0.4% 1.0% 1.0% 94 Zemach et al., 2010
Pezizales Tuber melanosporum WGBS Fruitbody 12.823% 32.2% 11.6% 13.7% 79 Montanini et al., 2014
Free-living mycelia 11.856% 32.4% 10.1% 13.0% 83
Ascobolus immersus RE/Southern blot; WGBS Mycelia NA NA NA NA NA Goyon et al., 1996
Phymatotrichum omnivorum HPLC Mycelia; Dormant sclerotia 1.53% NA NA NA NA Jupe et al., 1986
Pleosporales Cochliobolus heterostrophus RE/Southern blot Conidia NA NA NA NA NA Keller et al., 1991
Ophiostomatales Ophiostoma novo-ulmi HPLC Mycelia; yeast stage 1.38% NA NA NA NA Binz et al., 1998
Taphrinomycotina Schizosaccharomycetales Schizosaccharomyces pombe LC-MS/MS Yeast cell - NA NA NA NA Capuano et al., 2014
Yarrowia lipolytica GC/MS Yeast cell 0.36% NA NA NA NA Tang et al., 2012
Saccharomycotina Saccharomycetales Saccharomyces cerevisiae HPLC; LC-MS/MS Yeast cell - NA NA NA NA Binz et al., 1998; Capuano et al., 2014
Candida albicans GC/MS Mycelial-form cell; yeast-form cell 0.08% NA NA NA NA Russell et al., 1987
Mucoromycota Mucoromycotina Mucorales Phycomyces blakesleeanus WGBS Mycelia 0.94% 2.2% 0.3% 0.5% 60 Zemach et al., 2010
Phycomyces blakesleeanus RE-NNA Mycelia 0.48% NA NA NA NA Antequera et al., 1984, 1985
Spore 2.90% NA NA NA NA Antequera et al., 1984, 1985
Mucor rouxii RE; AFLP Ungerminated spores, spherical (phase I) spores, phase II spores (germlings) NA NA NA NA NA Cano et al., 1988; Reyna-Lopez et al., 1997
Myxomycota Physarales Physarum polycephalum HPLC Spherules 5.42% NA NA NA NA Fronk and Magiera, 1994

WGBS, Whole Genome Bisulfite Sequencing; MSRE, Methylation-sensitive restriction enzyme; RE-NNA, Restriction enzyme and nearest-neighbor analysis; AFLP, Amplified Fragment Length Polymorphism; HPLC, High-performance liquid chromatography; GC/MS, Gas chromatography-mass spectrometry; LC-MS/MS, Liquid chromatograph/tandem mass spectrometer. –, Not-detectable; NA, Not available. aVegetative: mycelia, conidia. bPre-sexual: mycelia, conidia, and protoperithecia.