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. 2021 Jan 22;11:608223. doi: 10.3389/fimmu.2020.608223

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Copyright © 2021 Imhof, Balic, Heilig, Chiquet-Ehrismann, Chiquet, Niehoff, Brachvogel, Thesleff and Koch

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Soft food diet does not revert the TNN-/- periodontal defect μCT cross-sectional scans at the height of the first molar showed incisor pulp obliteration in both 6 month old wildtype and tenascin-W/TNN deficient mice (A, B, asterisk). The wider periodontal space, indicated by an arrow (A and B, arrow) is still found in soft diet fed 6 month old tenascin-W/TNN deficient mice. Immunofluorescence stainings (C´, D´) confirmed the presence of branched nerve fibers (CD31 in red, smooth muscle actin (SMA) in white, Tuj1 in green) in tenascin-W/TNN deficient periodontal space. (scale bar μCT: 1 mm, mice, n = 6, histology: (C, D) 200 μm, (C´, D´) 50 μm).