Figure 4.

Oxidative burst of peripheral neutrophils was not affected by dLAN. Neutrophils were stimulated by phorbol myristate acetate (PMA) in the whole blood collected at ZT9 and ZT21 from rats exposed to either the control LD regime (CTRL) or dLAN (~2 lx) for 5 weeks. Production of reactive oxygen species (oxidative burst) was analyzed using 2′,7′-dichlorodihydrofluorescein diacetate by flow cytometry. (A, B) Representative flow cytometric dot plots showing unstimulated (A) and PMA stimulated (B) neutrophils (gated population in red). Cell frequencies are displayed next to the gate. (C) Quantification of oxidative burst, which was calculated as a fold increase in the median dichlorofluorescein (DCF) fluorescence of the PMA stimulated over the unstimulated neutrophils. Data represent the mean ± SEM. Black dots indicate the individual data points. Two-way repeated ANOVA was used for analysis. ***P < 0.001 indicates significant difference between ZT9 and ZT21.