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. 2020 Dec 21;12(3):372–381. doi: 10.1111/1759-7714.13759

Figure 2.

Figure 2

Identification of FOXP4 as a miR‐3180‐3p target gene. (a) The wild‐type and mutated binding site between miR‐3180‐3p and FOXP4. (b, c) A549 and H460 cell lines were cotransfected with miR‐NC and miR‐3180‐3p with a WT and MUT 3′UTR. Luciferase activity was measured after 24 hours cotransfection. A549: Inline graphic, miR‐NC; Inline graphic, miR‐3180‐3p. H460: Inline graphic, miR‐NC; Inline graphic, miR‐3180‐3p. (d, e) A549 and H460 cells were transfected with miR‐NC or miR‐3180‐3p mimics for 48 hours. The mRNA and protein levels of FOXP4 were analyzed using qRT‐PCR and western blotting Inline graphic, A549; Inline graphic, H460; Inline graphic, miR‐NC; Inline graphic, miR‐3180‐3p. (f, g) The mRNA and protein levels of FOXP4 were analyzed using qRT‐PCR and western blotting in HBE, A549 and H460 cells. (h) FOXP4 mRNA expression levels were extracted from The Cancer Genome Atlas (TCGA). *P < 0.05, **P < 0.01, and n ≥ 3.