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. 2020 Dec 7;32(2):597–600. doi: 10.1021/jasms.0c00341

Figure 1.

Figure 1

Theoretical temperature dependence on back-exchange of frozen HDX samples. The deuterium retention for an average protein backbone amide is shown after 24 h (blue) or 1 week (red) when stored at different temperatures. Notably, −60 °C is sufficient for maintaining >99% of deuterium after 24 h (blue).