Figure 3.

Performance assessment of the sample manager and automated HDX platform. (A) Deuteration levels of internal standard Bradykinin remain consistent for each sample stored in the dry ice/ethanol sample manager until total depletion of the dry ice reservoir occurs after 18 continuous hours. (B) Collection of discrete data sets was analyzed to compare variability between automated and manual thaw/inject methods. Within each data set, a fully deuterated reporter peptide was used to assess variability in back-exchange. The average deviation (x) for the automated and manual method was determined to be 0.051 and 0.060 Da, respectively. Details are shown in Table S1.