Figure 6.

Histopathology and hypercalcemia in allografted mice. All cell lines grown in nude mice. A and B: In the subcutis, T94 (A) and 501 (B) cells are spindle-shaped, with minimal to no neutrophilic infiltrate. C: Tom3 cells are large and round, with vesicular nuclei and abundant neutrophilic infiltrate. D: Spleen with an s.c. 501 tumor has no increase in myelopoiesis. Inset shows red pulp with erythrocytes, lymphocytes, erythroid precursors, and a megakaryocyte. E: Spleen with an s.c. Tom3 tumor has marked red pulp expansion. Inset shows red pulp with large numbers of mature and immature granulocytes, larger myeloid precursors, and a megakaryocyte. F: Lung metastasis is present only in mice with intratibial T94 cells. Unlike the s.c. tumor, the metastases have moderate neutrophilic infiltration (inset). G: Mice injected at any site with T94 and 501 cells have significantly higher plasma calcium levels than do Tom3-injected mice. Data are expressed as means ± SD. ∗∗P < 0.01 versus Tom3. Original magnification: ×40 (A–C); ×4 (D–F); ×20 (D–F, insets).