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. 2021 Feb 4;22:10. doi: 10.1186/s12860-021-00348-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Luciferase activity assay after overexpression of miRNA precursors. HEK393T cells were co-transfected with recombinant plasmids, which included MSI1 3’UTR downstream humanized Renilla luciferase reporter gene (hRluc) and miRNAs precursors. Also, HEK393T cells co-transfected with mutated Msi1 3’UTR for each miRNAs binding site downstream of hRluc luciferase reporter gene. For negative control analysis, HEK393Tcells co-transfected with a scrambled negative control miRNA (Control miRNA) and wild Msi1 3’UTR downstream hRluc. Then luminescence was measured. Error bars denote S.E.M. for three independent repeats in each experiment. Independent student t-test indicated differences between (*p < 0.05; **p < 0.01)