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. 2021 Feb 5;12:111. doi: 10.1186/s13287-020-02091-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — ASC-EVs alleviate cerebral ischemic injury through miR-22-3p. a RT-qPCR analysis of miR-22-3p expression in neurons. b RT-qPCR analysis of miR-22-3p expression in cerebral tissues. c Representative microscopic images of FITC-miR-22-3p mimics in neurons (× 400). d RT-qPCR analysis of miR-22-3p expression in neurons upon addition of RNase A in CM. e RT-qPCR analysis of miR-22-3p expression in neurons upon treatment of actinomycin D and ASC-EVs. f RT-qPCR analysis of miR-22-3p expression in ASCs upon miR-22-3p inhibitor. g RT-qPCR analysis of miR-22-3p expression in neurons incubated with the EVs from ASCs transfected with miR-22-3p inhibitor. h CCK-8 assay detection of neuron viability upon treatment of miR-22-3p inhibitor. i Flow cytometry detection of apoptosis upon treatment of miR-22-3p inhibitor. *p < 0.05, **p < 0.01, and ***p < 0.001. Measurement data were presented as mean ± standard deviation. The data among multiple groups were analyzed by ANOVA