Fig. 6.

KDM6B promotes the expression of BMP2/BMF axis. a Western blot analysis of the expression of KDM6B, BMP2, and BMF in neurons upon treatment of OE-KDM6B or si-KDM6B. *p < 0.05, **p < 0.01, and ***p < 0.001. ^#p < 0.05 vs. si-NC treatment. ^##p < 0.01 and ^###p < 0.001. b Western blot analysis of the expression of KDM6B, BMP2, and BMF in neurons upon co-culture with ASC-EVs or PBS. *p < 0.05 vs. PBS, **p < 0.01, and ***p < 0.001. c RT-qPCR analysis determining the transfection of OE-BMP2 in neurons. d Western blot analysis of KDM6B, BMP2, and BMF expression in neurons upon treatment of OE-BMP2, OE-NC, si-KDM6B, or si-NC. *p < 0.05, **p < 0.01, and ***p < 0.001. ^#p < 0.05 vs. si-KDM6B + OE-NC treatment. ^##p < 0.01 and ^###p < 0.001. e ChIP assay detection of the binding between KDM6B and BMP2 enhancer region or H3K27me3 modification upon treatment of si-KDM6B. *p < 0.05 vs. si-NC treatment, **p < 0.01, and ***p < 0.001. Measurement data were presented as mean ± standard deviation. The data between two groups were analyzed by an independent t test. The data among multiple groups were analyzed by ANOVA