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. 2021 Feb 5;12:111. doi: 10.1186/s13287-020-02091-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — ASC-EV-miR-22-3p suppresses KDM6B-mediated BMP2/BMF axis and alleviates ischemic injury. a RT-qPCR analysis determining the efficiency of si-BMF (siRNA1, siRNA2, and siRNA3). b Western blot analysis determining the efficiency of si-BMF (siRNA1, siRNA2, and siRNA3). *p < 0.05 vs. si-NC, **p < 0.01, and ***p < 0.001. c RT-qPCR analysis of miR-22-3p, KDM6B, BMP2, and BMF mRNA expression in neurons upon treatment of si-BMF and EVs. ***p < 0.001 vs. EV-inhibitor NC + si-NC and ^###p < 0.001 vs. EV-miR-22-3p inhibitor + si-NC. d Western blot analysis of miR-22-3p, KDM6B, BMP2, and BMF expression in neurons upon treatment of si-BMF and EVs. e CCK-8 assay detection of viability upon treatment of EV-inhibitor NC, siBMF, EV-miR-22-3p inhibitor, and si-NC. f Flow cytometry detection of apoptosis in neurons upon treatment of EV-inhibitor NC, si-BMF, EV-miR-22-3p inhibitor, and si-NC. g Cerebral infraction volume with TCC staining. h NeuN immunofluorescence of positive NeuN cells upon treatment of ASC-EVs, PBS, and sham operation. i TUNEL staining of neurons apoptosis upon treatment of ASC-EVs, PBS, and sham operation. From f to i, *vs. EV-miR-22-3p inhibitor + NC: *p < 0.05, **p < 0.01, and ***p < 0.001; ^#vs. EV-miR-22-3p inhibitor + si-NC: ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001. Measurement data were presented as mean ± standard deviation. The data between two groups were analyzed by independent t test. The data among multiple groups were analyzed by ANOVA