Table 3.

Box-Behnken design (matrix and responses) for levan production by BD1707

Run	A	B	C	D	Levan production (g/L)
					Experimental	Predicted
1	−1	0	1	0	31.72	31.82
2	0	0	0	0	33.40	33.36
3	0	0	−1	−1	21.92	22.47
4	1	0	−1	0	33.16	32.87
5	0	−1	0	−1	11.46	10.30
6	1	1	0	0	27.88	28.47
7	0	0	0	0	32.94	33.36
8	0	0	1	1	30.97	30.62
9	−1	0	−1	0	27.28	28.42
10	0	1	0	−1	19.94	19.84
11	0	1	−1	0	28.77	27.76
12	1	0	1	0	31.44	30.10
13	0	1	1	0	27.95	27.50
14	−1	−1	0	0	17.60	17.21
15	−1	0	0	1	29.96	29.69
16	0	−1	−1	0	16.84	17.29
17	−1	1	0	0	27.36	27.37
18	1	0	0	1	31.80	32.38
19	1	−1	0	0	18.64	18.84
20	0	1	0	1	27.08	28.04
21	0	0	1	−1	22.30	23.34
22	1	0	0	−1	22.96	23.22
23	−1	0	0	-1	23.76	23.17
24	0	-1	1	0	17.18	18.18
25	0	-1	0	1	17.88	17.79
26	0	0	0	0	33.74	33.36
27	0	0	-1	1	31.71	30.87